Zymographic Analysis of Maize Peroxidase Isoenzymes in Response to Fungicide Stress

Author: Shobha N. and Savitha G.

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Abstract

The peroxidase isoenzymes have essential role in the oxidative protection of plants under abiotic stress. The isoenzymes are a set of proteins catalysing the same reaction but they differ in number of properties. Analysis of these isoenzymes helps in finding the mutagenic potential of plants in response to environmental conditions. The native PAGE analysis was conducted which separates the isoenzymes based on their electrophoretic mobility and then visualized using staining solution containing substrate. The experiment showed Up-regulations of few isoenzymes and also down-regulations of some of the isoenzymes is observed in different concentrations of the fungicide i.e., metalaxyl treatment. The study shows the induced peroxidase is Ca2+ dependent. However, inclusion of EGTA could not effectively inhibit the peroxidase activity which clearly shows the involvement of multiple signal pathways.

Keywords

Peroxidase, isoenzymes, native PAGE, EGTA

Conclusion

Zymography of peroxidases studied on 0, 5th and 7th day of germination showed the isoenzyme pattern. Some of the isoenzymes were up-regulated whereas others are down-regulated, depending on the stress level. The expression of isoenzymes was more intense at higher concentration of metalaxyl on 7th day of germination. And the zymogram also revealed that induced peroxidase is calcium dependant but the presence of EGTA could not inhibit enzyme activity effectively. This may suggest the involvement of multiple signalling pathways. These results further confirms our findings on the expression of isoforms of peroxidase depends on the growth stage which may be due to expression of different genes.

References

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How to cite this article

Shobha N. and Savitha G. (2023). Zymographic Analysis of Maize Peroxidase Isoenzymes in Response to Fungicide Stress. Biological Forum – An International Journal, 15(5): 1781-1787.