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Molecular profiling of a novel bacteria Cronobacter sakazakii (ON248143), degrading organophosphate (MCP) Pesticide from Contaminated Soil of Agricultural land

Author: M. Chidambarapriya, P. Vanitha Pappa and K. Madhubala

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Abstract

The widespread and indiscriminate use of pesticides in agriculture for pest control has caused major harm and issues for both humans and biodiversity. It has been thought to be useful to cleanse areas that have been contaminated by pesticides via microbial biodegradation of pesticides in contaminated soils. A commonly used organophosphorus insecticide called monocrotophos has negative consequences, particularly those on the central nervous system. This study's objective was to use an enrichment culture approach to isolate bacteria from Monocrotophos (MCP)-contaminated soil. The isolates received a high dose (1000 ppm) of MCP as a carbon source supplement. Based on the 16S rRNA gene sequence, the isolate was determined to be Cronobacter sakazakii (ON248143), a microorganism that may degrade MCP. The outcomes were validated with an analytical tool, High-Performance Liquid Chromatography (HPLC).

Keywords

Pesticides, central nervous system, organophosphorus, enrichment culture technique, HPLC

Conclusion

With this increased awareness, research has recently focused on the fate of pesticides in soils and health risks due to their transfer and accumulation in plants. Our study reveals that isolated bacterial strains growing in contaminated sites can withstand high levels of monocrotophos in the soil. The potent isolated bacteria in Ops biodegradation were molecularly identified as Cronobacter sakazakii (ON248143) by 16S rRNA gene sequence. New strain Cronobacter sakazakii(ON248143) proved that is a good tool in the bioremediation process in MCP contaminated soil.

References

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How to cite this article

M. Chidambarapriya, P. Vanitha Pappa and K. Madhubala (2023). Molecular profiling of a novel bacteria Cronobacter sakazakii (ON248143), degrading organophosphate (MCP) pesticide from contaminated soil of agricultural land. Biological Forum – An International Journal, 15(4): 584-589.